The recombinant vector pqe - 80l / dhfr / hpd - 3 was identified by restriction digestion while we also construct the recombinant control vector pqe80l / dhfr with the same strategy . 3 同時還將表達運載蛋白dhfr的基因單獨連接于表達載體pqe - 80l ,得到對照質(zhì)粒pqe - 80l dhfr 。
So we present two methods : the mod method by introducing mismatched control vector and the ssp algorithm by partitioning subspaces to reduce the dimensions of adaptive process 為此,我們提出了兩種方法:通過引入失配方向控制向量而得到的最優(yōu)失配檢測方法和通過子空間劃分而減小自適應(yīng)維數(shù)而得到的子空間投影方法。
( 2 ) the construction of the recombinant prorarylic expression vector pqe80l / dhfr / hpd - 3 and the control vector pqe80l / dhfr . an about 135 bps fragment could be seen after pqe80l / dhfr / hpd - 3 vector was digested by kpn i + hindiii Sds一隊ge分析可見重組原核表達載體pqe一80l / dhf側(cè)hpd一3表達的目的蛋白帶和對照質(zhì)粒pqe一sol / dhfr表達的蛋白帶,大小分別約為31kd和26kd 。
2 . to construct the prokaryotic expression vector and the control vector . after sequenced , the target dna fragment was cloned into pqe - 80l vector together with the dna fragment encoding carrier protein dhfr 將自puc18 h d - 3回收的目的片段連同表達運載蛋白dhfr的基因片斷一起連接于原核高效表達載體pqe - 80l ,經(jīng)酶切鑒定,得到重組原核表達載體pqe - 80l dhfr h d - 3 。
Discovery could quickly trigger measures to control vector proliferation without harming the environment , to advise the public about self - protection , to provide vaccines ( when available ) for at - risk populations and to deliver prompt treatments 一旦發(fā)現(xiàn),就會快速采取措施在不破壞環(huán)境的情況下控制帶菌媒介的繁殖,建議公眾加強自我保護,為有危險的人群提供疫苗(有可能的話)以及及時的醫(yī)治。
Optimal predictive control to damp oscillation for linear time - delay systems with external sinusoidal disturbances is considered in chapter 4 . a full - order state predictive observer for time - delay systems by using the control vector and the predictive output vector of the controlled plant is structured 利用構(gòu)造預(yù)測模型得到的被控對象預(yù)測輸出向量和系統(tǒng)的控制向量,設(shè)計一種全維狀態(tài)預(yù)測觀測器,并將該狀態(tài)觀測器用于時滯控制系統(tǒng)的最優(yōu)前饋-反饋預(yù)測控制中。